Allied Innovative Systems, LLC

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Ultrasound Particle Agglutination (UPA) method

Ultrasound Particle Agglutination (UPA) is a platform technology in which ultrasound is used to improve the performance of conventional particle agglutination immunoassays. UPA improves conventional particle agglutination and haemagglutination assays, by increasing sensitivity, decreasing incubation times, lowering reagent costs and delivering more quantitative results.

Other ultrasound-based technologies have been applied to conventional particle agglutination assay in the past, but ALLIS technology is different and superior because UPA both enhances specific agglutination and decreases non-specific aggregation in a one-step procedure. In addition, ALLIS system provides for quantitative assessment of aggregates using simple optical means while samples remain in the resonator cell. These innovations allowed us to develop a one-step sensitive, simple and rapid method for detection of various physiologically active substances such as proteins (antigens or antibodies), bacteria and cells. Since UPA offers crucial advantages over other rapid tests, we believe that our ultrasound particle agglutination technology has great potential to meet the need for Point-of-Care (POC) sensitive diagnostics in different media including food, environmental and clinical samples.

As a platform technology, UPA presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silica microparticles, and biological cells. UPA is easily adapted to detect any target molecules for which antibodies can be raised. Comparison tests of the UPA to the to conventional particle agglutination assays have demonstrated a 20 fold increase in sensitivity for haemagglutination assays and a 30- to 1000-fold increase in the sensitivity of determination of HIV antibodies and food-borne pathogenic bacteria such as E.coli and Listeria monocytogenesis, respectively. In particular, with respect to HIV testing, we have determined that UPA is approximately 20-fold more sensitive than immunochromatography tests for detection of HIV antibodies.

UPA Key Features:

  • Enhancement to conventional technology
  • One-step 1-3 min test (over 10x faster then current)
  • Up to 1000-fold increased sensitivity
  • Small several microliter samples
  • Objective, quantitative determination of the analyte concentration
  • Applicable to wide range of targets
  • Point-of-care (POC) or automation use
  • Supported by the NIH funding
  • Novel patented technology

The UPA immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. The volume of sample needed for the test can be less than 10 microliters and testing takes only minutes. The UPA immunoassay consists of mixing sample containing an analyte to be detected with sensitized latex particles or red cells, and placing the mixture into the ultrasonic resonator cell of the UPA device. In the UPA device, the mixture is subjected to ultrasonic exposure, and the formation of aggregates is detected. The quantitative results are displayed on an LCD display.

Unlike conventional agglutination assays, UPA is a simple one-step assay. The only action that needs to be performed by the operator is to load the reagent mixture into the device. The quantitative test result can be seen on the device display or computer screen in approximately 1-3 minutes from start of the reaction. Thus, the subjective evaluation of the test results is eliminated, sensitivity is improved, and testing times are shortened vis a vis conventional assays.

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References:

  1. N. Ossina, N. Panikov, M. Sizova, S. Bystryak. ULTRASONIC IMMUNO-AGGLUTINATION TESTS FOR FOOD-BORNE PATHOGENIC BACTERIA. Proceedings of 19th INTERNATIONAL CONGRESS ON ACOUSTICS, MADRID, 2-7 SEPTEMBER 2007, pdf
  2. N. Ossina, F. Ketema, N. Constantine, S. Bystryak HIV ANTIBODY DETECTION USING AN ULTRASOUND PARTICLE AGGLUTINATION METHOD Proceedings of 19th INTERNATIONAL CONGRESS ON ACOUSTICS, MADRID, 2-7 SEPTEMBER 2007. pdf
  3. S. Bystryak, N. Ossina, A. Sarvazyan. US patent No. 7,989,177 "A METHOD AND DEVICE FOR ULTRASOUND ASSISTED PARTICLE AGGLUTINATION ASSAY"